The effects of simvastatin and NONO on the proliferation of breast cancer cells / 肿瘤

ABSTRACT

Objective: To investigate the effects of simvastatin and non-POU-dormain-containing, octamer-binding protein (NONO) on the proliferation of breast cancer MCF-7 cells, and to explore its possible mechanism. Methods: The expression levels of NONO mRNA and protein and the proliferation of breast cancer MCF-7 cells after transfection with NONO gene-targeted small interfering RNA (siRNA) (siRNA-NONO) and the NONO over-expression recombination vector pcDNA4/TONONO were detected by real-time fluorescence quantitative PCR, Western blotting, and cell counting, respectively. The proliferation and expression levels of NONO mRNA and protein of breast cancer MCF-7 cells after treatment with simvastatin (20 μmol/L) were detected by cell counting, real-time fluorescence quantitative PCR, and Western blotting, respectively. The proliferation of MCF-7 cells after treatment with simvastatin in pcDNA4/TO-NONO transfection group was determined by cell counting. The expression level of 3-hydroxy-3-methylglutaryl-CoA reductase (HMCCR) mRNA and the concentration of cholesterol were examined by real-time fluorescence quantitative PCR and tissue total cholesterol assay, respectively. Results: The expression levels of NONO mRNA and protein of MCF-7 cells in siRNA-NONO transfection group were lower than those in siRNA negative control (siRNA-NC) transfection group (P < 0.01, P < 0.05). The expression levels of NONO mRNA and protein of MCF-7 cells in pcDNA4/TO-NONO transfection group were higher than those in the empty vector pcDNA4/TO transfection group (both P < 0.01). The ability of proliferation of MCF-7 cells in siRNA-NONO transfection group was lower than that in siRNA-NC group (P < 0.05), while the ability of proliferation of MCF-7 cells in pcDNA4/TO-NONO transfection group was higher than that in the empty vector pcDNA4/TO transfection group (P < 0.05). The ability of proliferation and the expression levels of NONO mRNA and protein of MCF-7 cells in simvastatin (20 μmol/L) treatment group were lower than those in the control group (MCF-7 cells without simvastatin treatment) (P < 0.05, P < 0.01, P < 0.05). The ability of proliferation of MCF-7 cells after treatment with simvastatin in pcDNA4/TO-NONO transfection group was lower than that in the group without simvastatin treatment (P < 0.01). The expression levels of HMCCR mRNA and cholesterol of MCF-7 cells in siRNA-NONO transfection group were lower than those in the siRNA-NC transfection group (all P < 0.01) Conclusion: Simvastatin can suppress the proliferation of breast cancer MCF-7 cells, and this effect may be related to the inhibition of NONO expression.