Inhibition effect of targeting fusion protein TAT-OSBP-MKK6(E) on human ovarian xenograft tumor in nude mice / 肿瘤

ABSTRACT

Objective: To investigate the inhibition effect of trans-activator of transcription (TAT)-ovarian cancer specific binding peptide (OSBP)-mitogen-activated protein kinase kinase 6 mutant (E) [MKK6(E)] on human ovarian xenograft tumor in nude mice and its possible mechanism. Methods: The subcutaneous xenograft tumor model of human ovarian cancer HO8910 cells in nude mice was established. Then the tumor-bearing nude mice were randomly divided into three groups treatment group [TAT-OSBP-MKK6(E) was administered intraperitoneally], negative control group (TAT-OSBP was administered intraperitoneally) and the blank control group (the saline was administered intraperitoneally). The tumor growth, tumor volume and body weight of nude mice of three groups were observed. The apoptosis and the expressions of proliferating cell nuclear antigen (PCNA) and p38 of xenograft tumors were examined by TUNEL method, immunohistochemistry and Western blotting, respectively. Results: As compared with the negative control and the blank control groups, the growth rate of xenograft tumor in the treatment group was decreased significantly (P 5). The apoptosis index (AI) value in the treatment group was higher than those in the negative control and the blank control groups (P 0.05). Conclusion: TAT-OSBP-MKK6(E) can inhibit the growth and the expression of PCNA in subcutaneous xenograft tumor of ovarian cancer in nude mice. This effect may be related to promoting apoptosis.