Effects of EGFL 6 gene silencing on proliferation and invasion of human melanoma cells / 肿瘤

ABSTRACT

Objective: To explore the effects of epidermal growth factor-like domain 6 (EGFL 6) gene silencing by specific small interference RNA (siRNA) on the proliferation and invasion of human melanoma cells. Methods: The double-strand siRNA targeting EGFL 6 gene (named as EGFL6-siRNA) was designed and synthesized. The EGFL6-siRNA was transfected into human melanoma A375 cells by liposome transfection method. The mRNA and protein expression levels of EGFL6 in A375 cells were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. The proliferation and invasion abilities of A375 cells were analyzed by cell counting kit-8 (CCK-8) and Transwell assay, respectively. Results: After EGFL6-siRNA was successfully transfected into human melanoma A375 cells for 48 h, the EGFL6 mRNA and protein expression levels in A375 cells were significantly decreased (both P < 0.01). The EGFL6-siRNA could significantly inhibit the proliferation of A375 cells at different time points (24-72 h) after transfection (all P < 0.01), and significantly inhibit the invasion of A375 cells (P < 0.01). Conclusion: The specific siRNA can effectively silence EGFL 6 gene expression in human melanoma A375 cells, and suppress the proliferation and invasion of melanoma cells.