Influence of TGSJ formulae-medicated serum on proliferation of breast cancer MCF-7 cells and its mechanism / 肿瘤

ABSTRACT

Objective: To investigate the effect of medicated serum containing Chinese medicinal formulae Tiaogan Sanjie (TGSJ) on proliferation of breast cancer MCF-7 cells in vitro , and to explore its possible mechanism. Methods: The medicated serum was prepared by using healthy male SD rats receiving TGSJ. The MCF-7 cells were divided into 3 groups blank control group (cultured with normal nutrient fluid), negative control group (cultured with serum without medication) and TGSJ group (cultured with the medicated serum). After culture for 72 h, the cell viability was determined by MTT method, the expression level of p16 mRNA was detected by using semiquantitative RT-PCR, and the DNA methylation level of p 16 gene was detected by fluorescent quantitative MethyLight-PCR. Results: After culture with the medicated serum for 72 h, the proliferation rate of MCF-7 cells was obviously declined, the mRNA expression level of p 16 gene was obviously increased, and the methylation level of p 16 gene was obviously declined, as compared with those of the blank control group and the negative control group (both P < 0.05). Conclusion: TGSJ can inhibit the proliferation of breast cancer cells, and this effect may be related to reversing the methylation of p 16 gene and up-regulating the expression level of p16 mRNA.