Effects of magnolol in combination with 5-FU on cell proliferation and SFRP-4 expression of human colon cancer HCT-8 cells / 肿瘤

ABSTRACT

Objective: To investigate the effects of magnolol in combination with 5-fluorouracil (5-FU) on the proliferation of human colon cancer HCT-8 cells and the expression of secreted frizzled-related protein-4 (SFRP-4). Methods: After treatment with magnolol and 5-FU alone or magnolol in combination with 5-FU, the proliferation of colon cancer HCT-8 cells was detected by MTT method, and the cell cycle distribution and the apoptosis of HCT-8 cells were examined by flow cytometry (FCM) and Hoechst 33258 nucleic acid staining, respectively. The expression levels of SFRP-4 and p-catenin proteins in HCT-8 cells were examined by Western blotting. Results: The proliferation of HCT-8 cells was inhibited by magnolol and 5-FU alone or mamgnolol in combination with 5-FU (all P 2/M phases were decreased in 5-FU alone group and the combination treatment group (both P < 0.05). The expression levels of SFRP-4 in HCT-8 cells in all three treatment groups were higher than that in the blank control group (without any treatment) (P < 0.05), while the expression level of p-catenin in HCT-8 cells in combination treatment group was lower than those in 5-FU and magnolol alone groups (P < 0.05). Conclusion: Magnolol in combination with 5-FU can enhance the inhibition effect on proliferation of human colon cancer HCT-8 cells, and arrest the cells at G0/G1 phase. This mechanism may be related to regulating the expressions of SFRP-4 and p-catenin proteins.