The biological function of miR-214 in colon cancer HCT116 cells / 肿瘤

ABSTRACT

Objective: To investigate the expression of miR-214 (microRNA-214) in different colon cancer cell lines and its effect on the proliferation and apoptosis of HCT116 cells. Methods: The expressions of miR-214 and PIM1 mRNA in different colon cancer cell lines were detected by real-time fluorogenic quantitative-PCR, and the relationship between miR-214 and PIM1 mRNA expression was evaluated. The miR-214 mimics was transfected into HCT116 cells by LipofectAMINE 2000, then the expressions of miR-214 and PIM1 mRNA levels were detected by real-time fluorogenic quantitative-PCR. The change of proliferation ablility of HCT116 cells was detected by MTT method and colony-formation assay. The flow cytometry was used to examine the changes of apoptosis and cell cycle distribution. Results: The expression of miR-214 was down-regulated in HCT116 cells. The expression of miR-214 was negatively correlated with the expression of PIM1 mRNA in colon cancer cells (r = -0.943, P = 0.016). After transfection with miR-214 mimics, the expression level of miR-214 was up-regulated as compared with no transfection. The high expression level of miR-214 increased in miR-214 mimics group compared with that in the negative control group (P < 0.01). The high expression level of miR-214 could significantly inhibit the expression of PIM1 mRNA. After transfection with miR-214 mimics, the number of colonies was decreased (P = 0.026 9), the cell growth was inhibited (P < 0.000 1), the apoptosis rate was increased (P = 0.010 4), and the proportion of G1-stage cells was decreased as well as the proportion of S-stage cells was increased. Conclusion: MiR-214 can inhibit the proliferation and promote the apoptosis of colon cancer HCT116 cells. MiR-214 may act as a tumor suppressor in colorectal cancer by inhibiting PIM1. Copyright © 2013 by TUMOR.