Inhibiting effects of tamoxifen alone and in combination with doxorubicin on the growth of human osteosarcoma cell line MG-63 / 肿瘤
Tumor
;
(12): 409-414, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-848998
ABSTRACT
Objective:
To compare the effects of combination of TAM (tamoxifen) and ADR (doxorubicin) with the respective effect of TAM or ADR alone on the growth of human osteosarcoma MG-63 cells.Methods:
The mRNA expression of ER (estrogen receptor) in MG-63 cells was detected by RT-PCR (reverse transcription-PCR). The morphological changes of MG-63 cells treated with TAM or ADR alone as well as the combination of them were observed under an inverted microscope, and the cell viability was tested by MTT colorimetric assay.Results:
The expressions of ERα and ERβ were found in MG-63 cells. The typical apoptotic change of MG-63 cells was observed in each drug intervention group but not occurred in the control group without drug intervention. MTT colorimetric analysis demonstrated that the proliferation inhibition rate of MG-63 cells in the combination group was significantly higher (even two-fold higher) than those in the single-drug groups (with the same dose or double dose) (P < 0.05).Conclusion:
ERα and ERβ are expressed in human osteosarcoma MG-63 cells. Both TAM and ADR alone can inhibit the proliferation of MG-63 cells, and the combination of the two can present more obviously inhibitory effect. One of the possible underlying mechanisms might be that TAM can enhance the sensitivity of MG-63 cells to ADR. Copyright © 2013 by TUMOR.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Tumor
Ano de publicação:
2013
Tipo de documento:
Artigo
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