Effects of bone morphogenetic protein 9 on the proliferation and apoptosis of breast cancer MDA-MB-231 cells / 肿瘤

ABSTRACT

Objective: To investigate the effects of bone morphogenetic protein 9 (BMP9) on the proliferation and apoptosis of breast cancer MDA-MB-231 cells in vitro and in vivo. Methods: MD-AMB-231 cells were infected with pAdtrack-CMV/BMP9 or pAdtrack-CMV/GFP adenovirus. The expression levels of BMP9 mRNA in MDA-MB-231/GFP and MDA-MB-231/BMP9 cells were detected by RT-PCR. The proliferation inhibitory rate and the apoptosis rate of breast cancer cells were determined by MTT assay, colony-forming assay and flow cytometry method. Then the breast cancer MDA-MB-231, MDA-MB-231/GFP or MDA-MB-231/BMP9 xenografts in nude mice were established. The volume of xenograft tumor was measured, and the apoptosis rate was detected by TUNEL assay. Results: There was no expression of BMP9 mRNA in MDA-MB-231 and MDA-MB-231/GFP cells, but the expression of BMP9 mRNA in MDA-MB-231/BMP9 cells was significant. The proliferation inhibitory rate of MDA-MB-231/BMP9 cells was higher than that of MDA-MB-231/GFP cells ( P<0.05). The colony-forming rate of MDA-MB-231/BMP9 cells was lower than those of MDA-MB-231/GFP and MDA-MB-231 cells ( P<0.05). The apoptosis rate of MDA-MB-231/BMP9 cells was higher than those of MDA-MB-231/GFP and MDA-MB-231 cells ( P<0.05). The xenograft tumor volume in the MDA-MB-231/BMP9 group was smaller than those in the MDA-MB-231/GFP and MDA-MB-231 groups ( P<0.001). The apoptosis index of xenograft tumor in the MDA-MB-231/BMP9 group was higher than those in the MDA-MB-231/GFP and MDA-MB-231 groups ( P<0.001). Conclusion: BMP9 can inhibit the proliferation ability and induce the apoptosis of MDA-MB-231 cells in vitro and in vivo.