A case-control study on the associations of ER codon 325 and PR +331G/A with the risk of breast cancer / 肿瘤

ABSTRACT

Objective: To investigate the association of estrogen receptor (ER) codon 325 and progestin receptor (PR) +331G/A gene with the risk of breast cancer and the influence of the two genes combined with the environmental risk factors on breast cancer. Methods: A case-control study was performed. Two hundred and six cases with newly diagnosed breast cancer were collected. Two hundred and fourteen healthy women were matched according to age frequency (± three years). The epidemiological data were collected by questionnaire using structured tables. The 3 mL venous blood was collected and the genotypes of ER codon 325 and PR + 331 G/A were determined by fluorescent quantitative PCR. Results: Compared with CC, the adjusted OR was 0.99 (95% CI 0.61-1.61) and 1. 13 (95% CIO. 62-2. 04) for CG genotype and GG genotype of ER-codon 325, respectively. The adjusted OR was 1.51 (95% CI 0.74-3.07) for GG genotype of PR + 331 G/A. No synergistic effects of ER codon 325 and PR + 331 G/A were observed in increasing the risk of breast cancer (Trend analysis, P = 0.409). The adjusted OR was 3.57 (95% CI 1. 21-10. 52) for the ER codon 325 CG- and GG-genotyped women with spontaneous abortion history compared with that in CC-genotyped women without spontaneous abortion history. Additive models test demonstrated that the difference tended to be significant (S = 3.06, AP = 0.97, u = 1.898 1 , P = 0.058 8). Conclusion: The single nucleotide polymorphism (SNP) of ER codon 325 and PR + 331 G/A genes are not associated with the risk of breast cancer. The SNP of ER codon 325 with spontaneous abortion history tends to synergistically increase the risk of breat cancer.