The expressions of CDC25A and CDC25B in esophageal squamous cell carcinoma and their relationship with cell apoptosis and proliferation / 肿瘤

ABSTRACT

Objective: To investigate the biological significance of the expressions of CDC25A and CDC25B and their relation to cell apoptosis and proliferation of esophageal squamous cell carcinoma. Methods: The expressions of CDC25A and CDC25B were examined by SP immunohistochemical stain (IHC) in 52 cases of esophageal carcinoma specimens, 25 cases of dysplasia and 24 cases of normal tissues. The apoptotic ratio, proliferation index (PI), the expression of CDC25A and CDC25B protein were determined by FCM in 30 cases with esophageal carcinoma and 5 cases of normal tissues. Results: The positive expression rates of CDC25A (50%) and CDC25B (48.1%) in esophageal carcinoma were significantly higher than those in dysplasia(16%) and normal tissues (0%) (P < 0.01); the expressions of CDC25A and CDC25B were correlated with differentiation degree and invasion depth of tumor cells, and CDC25A was related with lymph node metastasis of esophageal squamous cell carcinoma. The PI was significantly higher in nuclear membrane protein CDC25A-positive group than that in nuclear membrane protein CDC25A-negative group (P < 0.05). Conclusion: CDC25A is involved in the carcinogenesis and progression of esophageal squamous cell carcinoma and has the potential to become a new biological marker to predict the carcinogenesis and prognosis of esophageal carcinoma; CDC25B might play a role in the early phase of esophageal squamous cell carcinoma. The CDC25A protein in the nuclear membrane might contribute to cell proliferation.