Epidermal growth factor receptor mutation type III promotes the migration of glioma cells by increasing FAK phosphorylation / 肿瘤

ABSTRACT

Objective: To investigate the mechanism underlying the promoting effects of epidermal growth factor receptor mutation type III (EGFRv III) on the migration of glioma cells. Methods: Human glioma cell line U87EGFRv III was established by retroviral infection of the parental glioma cells. The effect of EGFRv III on the migration of the cells was detected by gel-drop assay and Transwell migration assay. The phosphorylation of FAK was detected by Western blotting. The FAK Y397F mutant was transfected into U87EGFRv III cells and the change in cell migration was observed. Results: EGFRv III promoted the migration of human glioma U87 cells and increased the FAK phosphorylation level at Tyr397. Transfection with FAK Y397F mutant reduced FAK phosphorylation level in glioma cells U87EGFRvH[ and induced the decrease in the migration abilities of the cells. Conclusion: EGFRv III promoted the migration of glioma cells by up-regulating FAK phosphorylation levels.