Inhibitory effects of periplocin from cortex periplocae on proliferation of human esophageal carcinoma TE-13 cells / 肿瘤
Tumor
;
(12): 203-206, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-849401
ABSTRACT
Objective:
To study the inhibitory effects of periplocin from cortex periplocae (CPP) on proliferation of human esophageal carcinoma TE-13 cells and the related mechanism for cell cycle arrest.Methods:
The inhibitory effects of CPP on the growth of TE-13 cells were measured by MTT assay. The morphological changes of TE-13 cells were analyzed by Gimsa staining. Detection of cell apoptosis and cell cycles were performed by flow cytometry. Protein expressions of cyclin-dependent kinase CDK2 and CDK4 were analyzed by Western blotting assay.Results:
CPP significantly inhibited the proliferation of TE-13 cells in a dose- and time-dependent manner (P 0.05). CDK4 expression was inhibites by CPP at different concentrations, but the expression of CDK2 had no marked changes (P > 0.05).Conclusion:
CPP significantly inhibites the growth of TE-13 cells. The action mechanism may be related with induction of cell cycle blocking and apoptosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Tumor
Ano de publicação:
2008
Tipo de documento:
Artigo
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