Correlation of the expression of pokemon with p14ARF and bcl-2 and their effects on prognosis of non small cell lung cancer / 肿瘤

ABSTRACT

Objective: To investigate the correlation of the expression of pokemon with p 14ARF and bcl-2 in non small cell lung cancer (NSCLC) tissues, and their values for determining the clinicopathological characteristics and prognosis of NSCLC. Methods: Immunohistochemical SP staining was used to detect the expressions of pokemon, p 14 ARF, and bcl-2 proteins in 62 NSCLC tumor tissues and 20 adjacent normal tissues. Their relationship with the pathological characteristics of NSCLC was analyzed. The correlation of abnormal expressions of pokemon and p 14 ARF and bcl-2 was analyzed. The effects of the expressions of the three proteins on the long-term prognosis of NSCLC patients were observed based on the follow-up data. Results: In normal tissues there was no pokemon expression but the positive rates of p 14 ARF and bcl-2 expression were 95.0% and 15.0%, respectively. In NSCLC tissues, the positive rate of pokemon, p 14 ARF,and bcl-2 protein were 72.6%, 66.1%, and 53.2%, respectively. The expression of pokemon had negative correlation with expression of p 14 ARF (r = - 0.287, P < 0.05), but had positive correlation with expression of bcl-2 (r = 0.293, P < 0.05). The expression of pokemon and p 14 ARF was associated with TNM staging (P < 0.05). However, the expression of bcl-2 correlated with pathological classification (P < 0.05). In pokemon and bcl-2 positive patients, the five-year survival rates were 10.95% and 13.74%, respectively, which were significantly lower than that in pokemon and bcl-2 negative patients (P < 0.05). In contrast, the five-year survival rate was 21.68% in patients with positive expression of p 14 ARF, which was significantly higher than that in patients with negative expression of p 14 ARF (P < 0.05). Conclusion: Pokemon, p 14 ARF, and bcl-2 are all expressed in NSCLC tissues. There is a negative correlation between the expression of pokemon and p 14 ARF and a positive correlation between the expression of pokemon and bcl-2, which has a certain clinical significance for evaluating the prognosis of NSCLC patients.