Effects of Nordy on proliferation, apoptosis, and differentiation of HPV 16 subgenes-immortalized human endocervical cells / 肿瘤

ABSTRACT

Objective: To investigate the effects of Nordy on proliferation, differentiation, and apoptosis of HPV 16 subgenes (E6,E7)-immortalized human endocervical cells (H8 cells). Methods: The inhibitory effects of Nordy on proliferation of H8 cells were measured by MTT assay. The expression of nuclear antigen mcm 5 in H8 cells was detected by immunocytochemical SP method. The effect of Nordy on cell cycle and apoptosis of H8 cells was analyzed by flow cytometry (FCM). Morphological changes of H8 cells were observed by light and electron microscopy. The activity of telomerase was tested by telomeric repeat amplification protocal-enzyme linked immunosorbent assay (TRAP-ELISA). Results: Nordy 10-100 μmol/L inhibited the proliferation of H8 cells to different extent, decreased the intracellular expression of mcm 5 protein, and arrested H8 cells in G0/G1 phase, reduced the proportion of H8 cells in S phase, and increased the apoptotic rate. Morphological examination showed that Nordy-treated H8 cells tended to differentiate into mature cells. The activity of telomerase decreased significantly after Nordy treatment. Conclusion: Nordy inhibites proliferation, activates apoptosis, and induces differentiation of H8 cells by blocking cell cycle and decreasing activity of telomerase.