Effect of STAT 5 decoy oligodeoxynudeotide on tumorigenicity of K 562 cells in nude mice / 肿瘤

ABSTRACT

Objective: As a potential gene therapeutic method, transcription factor decoy strategy has been used to block the transcription function of multi-transcription factors. This study aims to further verify the inhibitory effect of signal transducer and activator of transcription 5 (STAT 5) decoy oligodeoxynucleotides (ODN) on tumor growth in nude mice. Methods: The xenografted tumor model was established by subcutaneously injecting leukemia K 562 cells in nude mice. Liposome-ODN complex was directly injected into tumor body once daily every 3 days. The tumor growth and tumor size were measured everyday. Nude mice were sacrificed at the end of the experiment. The tumor was removed and weighed. RT-PCR and Western blotting were performed to detect mRNA and protein expression of bcl-xL, cyclin D1, and c-myc, respectively. Results: The tumorigenicity of K 562 cells was significantly inhibited by decoy ODN. The tumor weight was markedly reduced in decoy ODN group than mutant ODN group [(0.485 ± 0.178) g vs (0.928 ± 0.223) g, P < 0.05 ]. The tumor growth inhibition rate was 47.7%. RT-PCR and Western blotting showed that the mRNA and protein expression level of bcl-xL, cyclinD1, and c-myc were down-regulated. Conclusion: Transcription factor decoy strategy effectively blocks transcription of STAT 5 target genes in xenografted tumor of nude mice.