The effect of silencing HPV16-E6 oncogene by siRNA interference on cervical cancer cells / 肿瘤

ABSTRACT

Objective: We tried to construct and screen the most effective expression vector of siRNA targeting HPV-E6 oncogene and investigate its long-term influence on HPV-E6 gene expression in cervical cancer cells. Our aim is to discuss the molecular mechanism of E6 gene in the development of cervical cancer and explore the new approach to prevention of HPV infection and saving patients with cervical cancer. Methods: HPV16-E6 siRNA was transfected into CaSki cells mediated by FuGene 6. The expression of HPV16-E6 was detected by western blot; the influence of HPV16-E6 siRNA on cell proliferation was detected by MTT assay; the effect of HPV16-E6 siRNA on cell cycle and apoptosis was determined by flow cytometry. Results: HPV16-E6 siRNA significantly inhibited HPV16-E6 expression, suppressed the proliferation and cell cycle progression, and induced apoptosis of CaSki cervical cancer cells. Conclusion: HPV16-E6 siRNA effectively inhibited cell proliferation, arrested cells at G0/G1 phase, and induced apoptosis in CaSki cells.