DNA content, the expression of VEGF and p53 in pleural effusion can predict the cure and prognosis / 肿瘤

ABSTRACT

Objective: To investigate the association of nuclear DNA content and vascular endothelial growth factor (VEGF) and p53 expression with therapeutic response of malignant pleural effusion and their value in predicting the prognosis of wet lung cancer. Methods: The survival periods of 43 lung cancer patients with pleural effusions were followed up. The DNA content of 39 patients was measured by the image cytometry (ICM) and the expression of VEGF and p53 was determined by Envision immunohistochemical method. Twenty-nine patients were given bleomycin or interleukin-2 intrathoracically after drainage. Results: DNA aneuploid had a tendency to correlate with therapeutic efficacy of malignant pleural effusion (P = 0. 054). Cox multivariate analysis showed that only p53 expression was independent prognostic factor for lung patients with pleural effusion (P = 0.05). The median survival time of patients was (10.4 ± 3.5) months for p53-negative patients and (2.8 ± 0.6) months for p53-positive patients (log rank = 0.013 2). One-year survival rate was 17.7% for p53-negative patients and 0% for p53-positive patients. Conclusion: DNA content measured by ICM tended to correlate with the therapeutic efficacy of malignant pleural effusion; p53 expression is a unique independent prognostic factor for lung cancer patients with pleural effusions.