Dynamic expression changes of SOCS3 and pro-inflammatory factors in the early inflammatory response of severe acute pancreatitis in rats / 解放军医学杂志

ABSTRACT

Objective To investigate dynamic changes of SOCS3 and pro-inflammatory factors expression in the early inflammatory response of SAP to benefit early treatment of SAP. Methods A total of thirty-two male SD rats were randomized into control groups (NC groups), SAP groups 6 h, 12 h, and 24h. SAP was induced by retrograde infusion of 4% sodium taurocholate into the biliopancreatic duct. The serum level of amylase (AMY), creatinine (Cr), and urea nitrogen (BUN) were quantified. The histopathologic changes of pancreas and kidney were examined under a light microscope. The levels of TNF-α, IL-18, and IL-1β in serum and kidney were determined by ELISA and RT-PCR, respectively. The expression of SCOS3 in kidney was detected by RT-PCR and Immunohistochemical analysis. Results Compared with the normal control group, serum biochemical enzymes and inflammatory factors in SAP groups were increased significantly. In addition, SAP groups showed severe degrees of pancreatic and renal injury gradually (P<0.05). The expression of SOCS3 was significantly higher in SAP groups than the control group, which was consistent with the trend of proinflammatory factor expression (P<0.05). Conclusion In the early stage of SAP, the release of inflammatory factors exacerbates the injury of the pancreas and extra-pancreatic organs. SOCS3 is involved in the early organ injury of SAP, which may play an important role in regulating the inflammatory reaction.