Protective effects of astragalus-lily granules on intestinal mucosal barrier of mice in high altitude hypoxia / 解放军医学杂志

ABSTRACT

Objective To investigate the protective effect of Astragalus-Lily Granules on intestinal mucosa and intestinal flora homeostasis in mice under high altitude hypoxia condition. Methods We put mice into high altitude hypoxia cabin to establish high altitude hypoxia model mice. Sixty Kunming mice were randomly divided into control group, model group, Astragalus- Lily particles (ALP) low, medium and high dose groups [1.75, 3.5, 7g/(kg·d)] respectively. After three days of routine feeding, the ALP mice received drug by intragastric administration, once a day for continuous 17 days, control group and model group were given double distilled water in same volume. From the 15th day, all the mice but control group were exposed to simulated high altitude hypoxia condition for 3 days in a high altitude hypoxia cabin after they were gavaged for half an hour daily. By the 18th day, the fresh mouse feces were collected and smeared to observe the changes of microflora. The pathological changes of intestinal tissues were observed by HE staining and the expression of HIF-1α protein in intestines was detected by immunohistochemistry. Results The enterococci and gram negative bacteria showed a higher proportion (65.2%±2.4% and 56.7%±3.3%, respectively) in the model group compared with the control group (24.7%±1.2%, 23.2%±1.5%, respectively, P<0.05). The pathological score of intestinal mucosal necrosis and edema (3.10±0.99, 3.30±0.67 respectively) and inflammatory cell count (15.93±3.30, 16.40±3.97/ HP respectively) was higher compared with the model group (0.70±0.67, 0.80±0.78; 4.07±2.12, 4.28±2.16/HP respectively; P<0.05). HIF-1α expression increased significantly compared with the model group (P<0.05). The enterococci (46.7%±2.0%, 32.0%±2.6% respectively) and gram negative bacteria rate (34.2%±1.6%, 38.0%±2.8% respectively) in the ALP medium and high dose groups were lower compared with the model group (24.7%±1.2%, 23.2%±1.5% respectively, P<0.05). The pathological score of intestinal mucosal necrosis and edema in small intestine (2.30±1.33, 2.10±0.94 respectively) and colon (2.50±1.08, 1.90±0.99) were lower than those of model group and inflammatory cell count (small intestine 13.26±2.34, 10.93±3.67/HP, colon 14.40±2.02, 11.33±2.96/HP, respectively) were lower than those of the model group (P<0.05), and the HIF-1α expression in the intestinal tissues increased significantly compared with the model group (P<0.01). Conclusion ALP has certain protective effect on intestinal mucosa and microecology of mice under high altitude hypoxia condition.