Effect of miR-182 in non small cell lung cancer via regulating the reck signaling pathway / 解放军医学杂志

ABSTRACT

Objective To explore the effect of miR-182 in non small cell lung cancer (NSCLC) and its mechanism. Methods The expression level and difference of miR-182 were detected by qRT-PCR in NSCLC cell line and matched tissues of 30 patients suffering from NSCLC. Cell proliferation, invasion and metastasis of A549 and H1299 via miR-182 was detected by miR- 182 mimics. The predicted target gene (RECK) of miR-182 was identified via luciferase activation assay. The mRNA or protein expression of RECK after miR-182 mimic or inhibitor transfection was detected by qRT-PCR or Western blotting, respectively. Results Expression of miR-182 was significantly increased in NSCLC tissues and cell lines compared with that in the adjacent tissues (P<0.01), and it was correlated with the metastasis of NSCLC. The cell proliferation, invasion, and migration capacity was increased after miR-182 mimics transfection. Dual luciferase assay showed that miR-182 directly regulated the RECK translation level. RECK expression level was decreased after miR-182 mimics transfection in the A549 cells, while increased after miR-182 inhibitor transfection. Conclusion miR-182 promoted tumor growth and metastasis of NSCLC by down-regulating the RECK expression.