Influence of ulinastatin on the proliferation of vascular endothelial cells and IL-6 and TNF-α release under heat stress / 解放军医学杂志

ABSTRACT

Objective To explore the influence of ulinastatin on the proliferation of vascular endothelial cells and release of IL-6 and TNF-α under heat stress. Methods-HUVEC cells were divided into control, and ulinastatin of different dosage groups (1000, 2000, 3000, 4000, 5000, 6000, 7000U/ml), and CCK-8 assay was used to investigate the influence of ulinastatin on the proliferation of vascular endothelial cells. HUVEC cells were also divided into control, 43℃ heat stress, and 43°C heat stress plus ulinastatin groups. ELISA assay was used to determine the effect of ulinastatin on the release of IL-6 and TNF-α. Results-All the above mentioned doses of ulinastatin showed no significant inhibitory effect on proliferation of HUVEC cells (P>0.05). ELISA results showed that, compared with control group and 43°C heat-stress group, both the IL-6 and TNF-α releases were decreased at 0h in treatment group with ulinastatin (P<0.05). The IL-6 and TNF-α releases were much lower at 6h in the treatment group with ulinastatin than those undergoing 43℃ heat-stress only group (P<0.05). Conclusions-Various doses of ulinastatin have no effect on proliferation of endothelial cells. However, it can inhibit the IL-6 and TNF-α releases from HUEVC cells under heat stress.