Mechanism of the preventive effect of breviscapus on pathogenesis of acute kidney injury in rats with sepsis / 解放军医学杂志

ABSTRACT

Objective To investigate the inhibitory effect and its related mechanism of Erigeron breviscapus on pathogenesis of acute kidney injury (AKI) in rats with sepsis. Methods Thirty-six male Wistar rats were randomly assigned into control group, sepsis model group (CLP group), and breviscapine treatment group (treatment group, n=12 each). The sepsis model was reproduced by cecal ligation and puncture (CLP). The rats were sacrificed 24 h after operation. The levels of blood urea nitrogen (BUN) and serum creatinine (Scr) were assayed with enzymatic assays, endothelin 1 (ET-1) and inducible nitric oxide synthase (iNOS) with ELISA, renal malondialdehyde (MDA) and superoxide dismutase (SOD) activities with xanthine oxide method, and the degree of renal tissue injury by micros copic examination after HE staining Results Compared with the control group, the levels of Scr, BUN, ET-1, iNOS, MDA and iNOS in CLP groups significantly increased, while the SOD activities significantly decreased (P<0.05). Compared with the CLP group, the levels of Scr, BUN, ET-1, iNOS, MDA and iNOS were lower markedly in treatment group, while the SOD activity elevated markedly (P<0.05). Conclusions Breviscapine may reduce AKI in rats with sepsis, and the effect may be related to the improvement of renal microcirculation, reduction of antioxidant enzyme activity, and decrease in oxidation products.