Differential Effects of Green Tea Polyphenol in the gamma-irradiation Induced Human Leukemic and Lymphoblastic Cell Damage / 대한핵의학회잡지

ABSTRACT

PURPOSE: The green tea polyphenol (GTPP) has been known to exert antioxidant activity as a radical scavenger as well as cancer preventive and cancer growth inhibition effect. The aim of this study was to identify whether GTPP not only potentiate the growth inhibition effect in gamma-irradiated human cancer cell but also exert protection action for irradiated human normal cell. MATERIALS AND METHODS: GTPP (80% catechin including > 45% EGCG) added in the HL60, human leukemia, and NC37, human lymphoblast, before irradiation. After establishing the amount of GTPP and the dose of radiation, the cells were treated with the GTPP for 6 hours and irradiated with the determined doses. RESULTS: Viability when 10 mug/ml GTPP added before gamma-irradiation with 1 Gy to NC37 cells was not different in comparison with control but it when was irradiated with 3 Gy significantly different (1 Gy; P=0.126, 3 Gy; P=0.010). 20 mug/ml GTPP did not show significant difference in both NC37 cells irradiated with 1 Gy and 3 Gy (1 Gy; P=0.946, 3 Gy; P=0.096). Viabilities were significantly decreased with concentration of additional GTPP in HL60 with 1 or 3 Gy (1 Gy; 69.0+/-1.7% vs 42.4+/-1.3%, 3 Gy; 66.9+/-3.9% vs 44.2+/-1.6 %). CONCLUSION: In vitro study, we certified that when the cells were irradiated with dose below 3 Gy, GTPP provide not only anticancerous effect against cancer cells but also radioprotective effect in normal cells simultaneously. Theses results suggest the possibility that consumption of green tea could give the radioprotective effect and maximize the effect on internal radiation such as radioiodine therapy concomitantly.