Mechanism of Shuangdan Recipe on treatment of coronary heart disease based on network pharmacology / 中草药

ABSTRACT

Objective: To explore the active components and molecular mechanism of Shuangdan Recipe in the treatment of coronary heart disease by using network pharmacology. Methods: The active ingredients of Shuangdan Recipe were screened by the five principles of generic drugs. The target protein of Shuangdan Recipe active ingredient was obtained by TCMSP database and the target protein of coronary heart disease was found in OMIN database. The interaction network of active component-potential target and active ingredient-coronary heart disease target of Shuangdan Recipe was constructed by using Cytoscape software. The core target of the effect of Shuangdan Recipe on coronary heart disease was screened by network topological characteristics such as degree, median degree, and near center degree; The STRING database protein interaction analysis was performed; The zebrafish model was used to study the pro-angiogenesis effect of Shuangdan Granules. Results: From the TCMSP database, 39 active components such as tanshinone and paeonol and 205 protein targets were obtained from Shuangdan Recipe. A total of 111 core target genes such as IL6, MAPK1, TP53, AKT1, and VEGFA in the treatment of coronary heart disease were screened out. GO analysis results showed there were 19 bioactivity enrichments including RNA regulation, inflammatory response, G protein coupling, steroid hormone receptor activity, cell differentiation, and vasoconstriction. The DAVID database was used to enrich the relevant pathways, and the first 13 pathways including VEGF signaling pathway, FXO signaling pathway and ErBb signaling pathway were screened for coronary heart disease. Conclusion: The characteristics of multi-component, multi-target and overall regulation of Shuangdan Recipe were verified by network pharmacology. The main mechanism of Shuangdan Recipe in coronary heart disease was predicted, which provided theoretical basis for its active ingredient research and clinical application.