Effects of buddleoside on hypertensive vascular remodeling through Ang II/AT1 signaling pathway in aorta / 中草药

ABSTRACT

Objective: The aim of this paper was to observe the effects of buddleoside on hypertensive vascular remodeling through Ang II/AT1 signaling pathway in aorta. Methods: We used SHR model to examine the blood pressure, vertigo time, histomorphology, and collagen fiber distribution of the aorta, and evaluate whether buddleoside could ameliorate the hypertensive vascular remodeling in vivo. Meanwhile, abnormal proliferation and migration of VSMCs induced by Ang II in vitro was used to identify the mechanism. The anti-proliferation effect of buddleoside in VSMCs was observed using MTT assay and crystal violet assay. The anti-migration effect in VSMCs was observed using monolayer-wounding and boyden chamber transwell assay. Furthermore, the protein expression of Ang II, AT1, MMP-2, MMP-9, Src, p-Src, Syk, and p-Syk were examined. Results: The results showed that buddleoside could significantly decrease SBP, DBP, MBP, and vertigo time, and improve the thickened media aorta, hypertrophy and disordered arrangement of VSMCs, distribution of collagen fibers. Buddleoside could also inhibit the proliferation and migration of VSMCs, inhibit the ROS production, and reduce the protein expression of Ang II, AT1, MMP-2, MMP-9, Src, and p-Src. Conclusion: These data supported that buddleoside can ameliorate hypertensive vascular remodeling by inhibiting the proliferation and migration of VSMCs. Its mechanism is mediated by the regulation of Ang II/AT1 signaling pathway.