Determination of seven saponins components in Panacis Japonici Rhizoma with quantitative analysis of multi-components by single marker / 中草药

ABSTRACT

Objective: To establish quantitative analysis of multi-components by single marker (QAMS) method for simultaneous determining the content of seven saponins in Panacis Japonici Rhizoma, and to evaluate the adaptation and application of QAMS method in the quality control of Panacis Japonici Rhizoma. Methods: The relative factor (fs/i) of ginsenoside Rg1, Re, Rb1, pseudoginsenoside RT1, chikusetsusaponin IV and a were established by HPLC method with chikusetsusaponin V as internal standard, which were used to calculate the content of seven saponins in Panacis Japonici Rhizoma. Meanwhile, external standard method (ESM) was used to calculate the content of seven saponins. The difference between QAMS and ESM was analyzed to evaluate the accuracy of QAMS. Results: The fs/i of ginsenoside Rg1, Re, Rb1, pseudoginsenoside RT1, chikusetsusaponin IV and IVa were 1.286 7, 1.432 7, 0.966 6, 0.962 4, 1.207 2, and 0.938 4. There was no significant difference between the content of 10 batches of Panacis Japonici Rhizoma determined by QAMS and ESM. Conclusion: The established QAMS method is accurate and can be used for quantitative analysis and quality evaluation of the content of seven saponins in Panacis Japonici Rhizoma.