Study on diarylheptanoids from flowers of Juglans regia / 中草药

ABSTRACT

Objective: To investigate the diarylheptanoids from the flower of Juglans regia. Methods: The compounds were isolated and purified by various column chromatographies, and their structures were identified by physiochemical properties and spectroscopic data. And MTT assays were performed to detect the in vitro cytotoxicity of compounds. Results: A total of 11 diarylheptanoids were isolated from the EtOH extract of the flower of J. regia, which were identified as tsaokoarylone (1), rhoiptelol C (2), (3S,7S)-3,7-dihydroxy-1-(4-hydroxyphenyl)-7-(3-methoxy-4-hydroxyphenyl)-heptane (3), 3-hydroxy-1-(3-methoxy-4-hydroxyphenyl)-7- (4-hydroxyphenyl) heptane (4), 1-(4-hydroxyphenyl)-7-(3-methoxy-4-hydroxyphenyl)-4-heptene-3-one (5), juglanin A (6), 2-oxatrycyclo[13.2.2.13,7]eicosa-3,5,7-(20),15,17,18-hexane-10,16-diol (7), 2-oxatrycyclo[13.2.2.13,7]eicosa-3,5,7-(20),15,17,18-hexane- 10-one (8), pterocarine (9), (8R)-32-methoxy-2-oxa-1(1,3),3(1,4)-dibenzenacyclodecaphane-16,8-diol (10), and juglanin B (11). Conclusion: Compounds 1-2, 4-5, and 7-8 are isolated from this genus for the first time. Compounds 1-11 are isolated from this plant for the first time, and compound 8 inhibited the proliferation of HCT-116, HepG2, BGC-823, NCI-H1650, and A2780 cancer cell lines in vitro, with IC50 values of 3.56, 2.26, 1.39, 2.62, and 1.18 μmol/L, respectively.