Colonization investigation of arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE) in roots of Polygonatum kingianum and their correlations with content of main functional components in rhizomes / 中草药

ABSTRACT

Objective: To investigate the colonization of arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE) in the roots of Polygonatum kingianum planted at different areas, and explore the correlations between the colonization rate and the main functional components. Methods: Fresh roots of P. kingianum from five plots in Yunnan Province were taken as research objects. The alkali dissociation method was used to investigate the colonization of AMF and DSE in the roots of P. kingianum. Then the morphological structure was photographed. The content of P. sibiricum polysaccharides, diosgenin, total flavonoids and extractum were separately determined by phonel-sulfate method, colorimetry method of vanillin-acetic acid-perchloric acid, spectrophotometry method with rutin standard and hot dipping method with alcohol. The correlations between the colonization rates of AMF or DSE and four main functional components were analyzed by Pearson correlation coefficient method. Results: The average colonization of AMF and DSE in the five plots were 26.25%-57.54% and 31.67%-45.19%, respectively. The colonization rates of AMF and DSE from HHMZ was higher than the other four others. All of correlations among the colonization rates of AMF, DSE and their typical structure and the four main functional components in the rhizomes were positive correlation, in which the correlations between the content of polysaccharides, diosgenin or total flavonoids and the colonization rates of AMF or DSE were higher, and their correlation coefficient were respectively 0.838/0.887, 0.819/0.703, and 0.785/0.855 (AMF/DSE). Furthermore, the correlations between the content of polysaccharides and the colonization rates of AMF hypha or DSE were significant. In addition, there were high correlations among the colonization rates of AMF, DSE and their typical structures, as well as the content in pairs of four functional components. Conclusion: There were higher colonization rates of AMF and DSE in the fibrous roots of P. kingianum. The correlations between the colonization rates of AMF or DSE and each main functional component were positive. This study provides data support and experimental basis for the implementation of ecological planting of P. kingianum and the use of biological means to increase production and income.rates