Study on secondary metabolites of endophytic fungus Alternaria alternate from Paeonia lactiflora / 中草药

ABSTRACT

Objective This study focused on the secondary metabolites of endophytic fungus Alternaria alternate in Paeonia lactiflora. Methods Compounds were isolated from the EtOAc extract by chromatography technology and their structures were elucidated on the basis of comprehensive spectroscopic analysis. Results A total of 15 compounds were isolated and their structures were identified as methyl 4-acetamido-3-hydroxybenzoate (1), (E)-methyl5-hydroxy-3-methylpent-2-eno (2), isobenzofuranone A (3), 4-hydroxyacetophenone (4), 6-hydroxy-isosclerone (5), talarojlavone (6), 7-hydroxy-2-hydroxymethyl-5-methyl-4H-chromen-4-one (7), alternarienonic acid (8), 7-hydroxy-2,5-dimethyl-4H-1-benzopyran-4-one (9), 5-hydroxy-epialtenuene (10), alternariol (11), methyl 3-hydroxybenzoate (12), stemphyperylenol (13), altenusin (14), and altenuene (15). Conclusion Compounds 1, 3, 5-10, 12, and 13 are isolated from Alternaria alternatefor the first time.