Optimization of preparation process of Jianpi Yishen Pill based on pharmacodynamic screening and orthogonal test / 中草药

ABSTRACT

Objective To screen the preparation technology of Jianpi Yishen Pill (JYP) by pharmacodynamics screening, and optimize it by orthogonal test to determine the best extraction technology. Methods The chronic renal failure model in rats was established by 5/6 nephrectomy. Three technological routes of JYP were selected, including A (powder of medicinal materials and decocting with water), B (powder of medicinal materials, extraction of volatile oil, and decocting with water), and C (extraction of volatile oil and decocting with water), with the content of Cr and BUN in rats as index. The orthogonal test was optimized by using the content of astragaloside and the quality of solid as indexes, with water volum, decoction time, decoction freguency as factors. Results The pharmacological efficacy test showed that technology A could obviously improve the rat model of chronic renal failure, and it could be used as the molding technology of this prescription pill. In this technology, five herbs such as Atractylodis Macrocephalae Rhizoma were crushed into fine powder alone, and the optimal extraction condition of other medical materials such as Astragalus Radix was as follows 8-fold water per time, decocting 1 h, for two times. Conclusion JYP can obviously improve the content of Cr and BUN in rats model of 5/6 nephrectomy. The optimized preparation technology is stable and feasible, which can provide the basis for the follow-up development of JYP.