Preparation of diallyl trisulfide microemulsion and its effect on proliferation and migration of tumor cells / 中草药

ABSTRACT

Objective To prepare diallyl trisulfide microemulsion (DATS-MEs) and evaluate its effect on the proliferation and migration of tumor cells. Methods Using HS15 as emulsifier, PEG400 as co-emulsifier and DATS as the oil phase, water titration method was used to prepare DATS-MEs. The influence of DATS-MEs on the proliferation of mouse Lewis cancer LLC cells and mouse melanoma B16BL6 cells in vitro was evaluated by the tetramethyl azoline blue (MTT) methed; Wound healing assay was used to evaluate the effect of DATS-MEs on the migration of LLC and B16BL6 cells in vitro. Results The DATS-MEs obtained was stable, clear, transparent, and well dispersed. The particle size of DATS-MEs was (16.72 ± 0.22) nm, the polydispersity index was 0.03 ± 0.01 and the Zeta potential was (-4.98 ± 0.11) mV. The encapsulation efficiency (EE) of DATS in microemulsion was 82.89%, the drug loading (DL) was 6.28%. The solubility in water increased by about 2 367 fold compared with the free DATS. DATS-MEs and free DATS at concentration of 10, 30, and 50 μmol/L all can effectively inhibit the migration and proliferation of LLC cells and B16BL6 cells in a dose-dependent manner. DATS microemulsion was proved to be more effective than free DATS. Conclusion After assembly into DATS microemulsion, the stability and solubility in water of DATS was enhanced. The anti-proliferation and anti-migration on mouse Lewis LLC cells and mouse melanoma B16BL6 cells were significantly enhanced.