Preparation of galangin molecular imprinted polymers on silica gel surface and its adsorption properties / 中草药

ABSTRACT

Objective To prepare surface molecular imprinted polymers (MIP) of galangin by using surface molecular imprinting technique. Methods Galangin MIP was prepared by surface polymerization method at the surface of silica gel, which was modified with (3-aminopropyl) trimethoxysilane, by using galangin as the template molecule, methacrylic acid (MAA) as the functional monomer, and N,N′-methylenebisacrylamide (MBA) as crosslinking agent. The polymer was characterized by infrared spectroscopy and scanning electron microscopy. And its adsorption properties were studied by static and competitive adsorption method. Results The experimental research showed that the optimal preparation condition was that the molar ration of galangin to MAA was 1∶4, with molar ration of MAA to MBA 1∶7, reaction temperature 40 ℃ and reaction time 12 h. Infrared spectrum and scanning electron microscopy showed that MIP was successfully grafted on the surface of silica gel, and recognition holes and sites selectively appeared for galangin molecules. Adsorption experiments exhibited that MIP had specific recognition and good affinity for galangin molecules. Compared to the controls of breviscapine and luteolin, the selectivity coefficients of MIP to galangin were 11.2 and 5.3, respectively. Conclusion MIP has good recognition and high selectivity for galangin, which provides a new method for the separation and extraction of flavonoids from Chinese medicine.