Simultaneous determination of 10 components in Paidu Yangyan Capsules by HPLC wavelength change method / 中草药

ABSTRACT

Objective The contents of sennoside A, sennoside B, echinacoside, verbascoside, hesperidin, aloe-emodin, rhein, emodin, chrysophanol, and rheochrysidin in Paidu Yangyan Capsules (PYC) were determined by HPLC. The quality of different batches of psoralen were evaluated by chemical pattern recognition. Methods HPLC was established for the simultaneous determination of 10 components in 15 batches of PYC with variable wavelength detector, and the results were analyzed by principal component analysis and cluster analysis to comprehensively evaluate the difference in quality of PYC which is available in the market. Results The contents of sennoside A, sennoside B, echinacoside, verbascoside, hesperidin, aloe-emodin, rhein, emodin, chrysophanol, and rheochrysidin in PYC had good linear relationship in the ranges of 0.167 4—2.093 0, 0.093 5—1.177 0, 0.075 9—0.948 6, 0.049 9—0.623 7, 0.109 0—1.363 0, 0.033 5—0.418 4, 0.079 7—0.996 1, 0.013 9—0.174 3, 0.025 2—0.315 6, and 0.012 3—0.061 4 μg/mL, the average sample recovery rate range were 99.84%, 99.15%, 99.34%, 99.81%, 100.6%, 99.36%, 99.86%, 100.1%, 100.1%, and 99.76% (RSD < 2.0%), the detection limits were 0.64—2.18 ng/mL, quantitative limits were 3.19—9.55 ng/mL, the content of 10 active ingredients in 15 batches of samples respectively were 0.712 7—1.118 0, 0.403 9—0.522 0, 0.236 4—0.320 3, 0.671 1—1.183 0, 0.0580—0.1467, 0.108 7—0.2592, 0.0147—0.0501, 0.5173—0.5828, 0.2754—0.3051, and 0.1621—0.1853 mg/grain and there were some differences among the samples in different batches, mainly due to different rhubarb dosages. Conclusion The established method is simple, accurate and reproducible, and can be used to control the quality of PYC. It is suggested that the quality control of Rhei Radix et Rhizoma should be strengthened in the process of preparation production for providing a reference of PYC follow-up quality improvement.