HPLC fingerprint of fruits of Gardenia Fructus based on cluster analysis and principal component analysis / 中草药

ABSTRACT

Objective To establish an HPLC fingerprint method of Gardeniae Fructus which could provide the basis for its authenticity identification and quality evaluation. Methods HPLC method was adopted to construct the fingerprint of Gardeniae Fructus and its main confused species (G. jasminoides f. logicarpa and G. jasminoides var. grandiflora). HPLC-ESI-MS was applied to identify the common peaks. The cluster analysis (CA) and principal component analysis (PCA) were used to classify the samples. Results The HPLC fingerprint of Gardeniae Fructus was obtained with 17 common peaks, and 15 common peaks were identified using HPLC-ESI-MS. A total of 22 batches of the fruits of Gardenia were classified to Gardeniae Fructus, G. jasminoides Ellis f. logicarpa, and G. jasminoides var. grandiflora by CA and PCA. Conclusion The establishment of HPLC fingerprint of Gardeniae Fructus and the application of chemical pattern recognition can provide scientific basis for authenticity identification and quality control of Gardeniae Fructus.