Promotion of Ziyuglycoside I on white blood cells by autophagy pathway / 中草药

ABSTRACT

Objective: To study the mechanism of ziyuglycoside I (ZgI) increasing the number of white blood cells (WBC) in peripheral blood. Methods: KM mice were randomly divided into control group, model group, 3-methyladenosine (3-MA) group, ZgI group (20 mg/kg), and ZgI (20 mg/kg)+3-MA group. On the first day of experiment, myelosuppression mice were induced by ip cyclophosphamide of 120 mg/kg and were continuously gavaged with ZgI for 6 d. On the 6th day, the number of WBC and granulocytes were determined. The degree of hematopoietic stem cells (HSCs) autophagy was observed by transmission electron microscopy and immunofluorescence microscopy. The apoptosis rate of HSCs was determined by flow cytometry, and the expression of Atg5, Atg7, and Beclin-1 proteins were detected by Western blotting. Results: Compared with the model group and 3-methyladenine group, ZgI significantly increased the numbers of leukocyte and granulocytes (P < 0.05), it also significantly stimulated the autophagy of HSCs. Meanwhile, ZgI significantly up-regulated the Atg5, Atg7, and Beclin-1 (P < 0.01) proteins expression in HSCs. Conclusion: The results suggested that ZgI was an efficient autophagic activator, and the mechanism of increasing WBC might be related to the promotion of HSCs autophagy of myelosuppression mice.