Effects of lipid-rich food on lymphatic transport of puerarin microemulsion / 中草药

ABSTRACT

Objective To study the effects of lipid-rick food on lymphatic transport absorption of puerarin microemulsion based on the puerarin microemulsion prepared before. Methods Cycloheximide was used to block lymphatic transport of the microemulsion, and the concentration of puerarin in plasma was determined by HPLC. Bioavailability of blocking group and unblocking group were compared to calculate the proportion of puerarin absorbed by lymphatic transport. Results Administration of lipid did not enhance the proportion of puerarin absorbed by lymphatic transport, compared to administration of water as controls, but microemulsion formulation contribute to the improved lymphatic transport and bioavailability of puerarin. Besides, administration of lipid failed to further increase the lymphatic transport of puerarin microemulsion, but it was evidenced that microemulsion formulation could decrease the effects of lipid-rich food on bioavailability of oral drugs. Conclusion Lipid in food could not enhance the lymphatic transport of puerarin microemulsion and show no significant effects on the bioavailability.