PCA and TOPSIS analysis and determination of amino acids and nucleosides in seeds of Annona squamosa from different producing areas by QTRAP-UFLC-MS/MS / 中草药

ABSTRACT

Objective To establish a QTRAP-UFLC-MS/MS method for the determination of 15 kinds of amino acids and 10 kinds of nucleosides in the seeds of Annona squamosa from different producing areas, toanalyze and investigate the difference of the 25 components in samples from five habitats, and provide scientific basis for its comprehensive utilization. Methods The analysis was carried out on a Waters XBridge Amide column (100 mm × 2.1 mm, 3.5 μm) with elution by mobile phase of 0.2% formic acid in water (A)-0.2% formic acid in acetonitrile (B) at a flow rate of 0.6 mL/min. The column temperature was maintained at 30 ℃. The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode. The comprehensive evaluation of the seeds from A. squamosa in five habitats was carried out by PCA and TOPSIS analysis. Results All 15 kinds of amino acids and 10 kinds of nucleosides showed good linearity (r2 > 0.994 7). The RSDs of the precision, repeatability, and stability tests were less than 3.87%. The average recovery rates were in the range from 94.90% to 98.78%, RSDs were in the range from 0.84% to 3.01%. There are differences in the composition and content of amino acids and nucleosides in the seeds of A. squamosa from different habitats, among which Hainan produced the highest comprehensive quality. Conclusion The method is simple, accurate, and reliable, which can provide the reliable basis for quality evaluation of the seeds from A. squamosa, and samples from different habitats can be optimized according to the target component.