Analysis on effect of astragaloside IV on lifespan extension of Caenorhabditis elegans based on 1H-NMR metabolomics / 中草药

ABSTRACT

Objective To investigate the anti-aging effects and the changes of endogenous metabolites in Caenorhabditis elegans by using astragaloside IV (AS-IV). Methods Nematodes tissue were collected from control group, age group and AS-IV group to analyze the metabolic profile changes among different groups by nuclear magnetic resonance (NMR). Results Using 1H-NMR metabonomics and multivariate statistical analysis methods, the changes in the levels of endogenous metabolites in the model group C. elegans were obtained, including eight increasing amino acids isoleucine, leucine, valine, glutamate, glutamine, succinate, taurine and threonine and decreasing compound glucose, compared with the control group. AS-IV (5 μmol/L) can significantly regulate all of them. Conclusion AS-IV can regulate amino acid metabolism and energy metabolism to anti-aging. The metabonomic approach is an effective tool for the investigation of the pharmacologic mechanism of AS-IV and it is helpful for further research.