Investigation on positive effect and possible mechanisms of Lvjiao Buxue Granules treatment on cyclophosphamide-induced leucopenia mice through 1H-NMR based metabolomics approach / 中草药

ABSTRACT

Objective To investigate the positive effect and possible mechanism of Lvjiao Buxue Granules on the leukopenia induced by cyclophosphamide in mice using 1H-NMR metabolomics approach. Methods Cyclophosphamide was used to establish the leucopenia mice, and the content of white blood cells, red blood cells, hemoglobin, platelets and other routine blood indexes were measured. The changes of endogenous metabolites in serum were analyzed by 1H-NMR metabolomics to investigate the regulation effect of Lvjiao Buxue Granules on serum endogenous metabolites in mice with leukopenia and the regulation mechanism of metabolic pathway. Results Compared with the control group, the contents of white blood cells, red blood cells, hemoglobin, and platelets in the model group were decreased significantly (P < 0.01), while the level of white blood cells and platelets in the treatment group were increased significantly (P < 0.05), and Lvjiao Buxue Granules can call back the level of potential differences metabolites. Conclusion Lvjiao Buxue Granules can improve leukopenia by regulating the immune function, energy metabolism, and amino acid metabolism.