HPLC fingerprint of Hyoscyamus Extract Tablets and quantitative analysis of tropane alkaloids / 中草药

ABSTRACT

Objective: To establish a fingerprint of Hyoscyamus Extract Tablets (HET) by high performance liquid chromatography and determine the content of atropine, anisodamine, scopolamine, and anisodine. Methods: The separation was performed on a GL Sciences Inc C18 (250 mm × 4.6 mm, 5 μm) column with gradient elution of methanol and 0.05% phosphoric acid solution. The detection wavelength was set at 218 nm, the flow rate was 1.0 mL/min, and the column temperature was 30℃. Similarity evaluation system for chromatographic fingerprint of TCM was used for establishing the reference fingerprint and similarity evaluation, and clustering analysis was carried out by SPSS 19.0. Results: There were 14 marked common peaks, and the similarity of S1-S4, S11 and the control fingerprint was over 0.98, others were above 0.995. It was shown that these 12 batches of samples were of good similarity. Cluster analysis results showed that the S1-S6 were grouped into one class, and the remaining samples were grouped into one class. The results of assay showed that alkaloids in different batches of HET varied a lot in contents but kept consistent in compositions. Conclusion: The method could well display the chemical information of HET. It has simple operation and good repeatability, which provides more comprehensive and scientific data for quality evaluation of HET.