Comparison of chemical constituents in Hippocampus of different breeds by 1H-NMR metabonomics approach / 中草药

ABSTRACT

Objective To compare the chemical constituents in Hippocampus kelloggi, H. kuda and H. trimaculatus by 1H-NMR metabonomics approach, provides the basis for quality evaluation of Hippocampus. Methods NMR-based metabonomics approach was used to analyze three breeds of Hippocampus and identify the chemicals. Multivariate statistical analysis was used to find the difference and regularity in the comparison of chemical constituents in different breeds of Hippocampus. Results Thirty-three metabolites were identified in the NMR spectra with methanol-ultrapure water (11); The multivariate analysis revealed that there were little differences between H. kelloggi and H. kuda; Compared with them, H. trimaculatus has the lower level of isoleucine, methionine, glutamic acid, hydroxyproline, proline, arginine, and cysteine, and the higher content of taurine and glycerin, which existed significant difference. Conclusion Compared the chemical constituents of different breeds of Hippocampus from the overall level to find their otherness and regularity, providing a new idea to control the quality of Hippocampus.