Correlation analysis between genetic diversity and limonin compounds content of Citri reticulatae Semen / 中草药

ABSTRACT

Objective To study the correlation between the genetic diversity and limonin compounds content of Citri reticulatae Semen germplasm resources in Sichuan to provide a reference for screening the main source varieties of C. reticulatae Semen. Methods Firstly, NTSYSpc2.10e software was used to analyze the ISSR markers results of 35 C. reticulatae Semen samples from different habitats and different varieties in Sichuan. Then, UPLC was used to determine the contents of limonin compounds (limonin, nomilin, and obacunone). Finally, all the results were analyzed by SPSS 21.0 software. Results A total of 240 clear and detectable DNA bands were generated from 33 ISSR primers, and the variation range of genetic similarity coefficient were 0.72-0.92. Citrus reticulate ‘Ponkan’ and C. reticulate ‘Dahongpao’ have the closest relationship and their Limonin compounds contents were similar, but the relationship between other varieties and Citrus reticulate ‘Dahongpao’ was far and limonin compounds contents had great difference. Conclusion The relationship of variety is closer to the C. reticulata ‘Dahongpao’, the content of limonin compounds is higher, and Citrus reticulate ‘Ponkan’ could be considered as the main source variety of Citri reticulatae Semen.