Simultaneous determination of nine components in Paeoniae Radix Rubra by HPLC / 中草药

ABSTRACT

Objective A high performance liquid chromatographic method (HPLC) was established to simultaneously quantify the paeoniflorin, oxypaeoniflora, albiflorin, benzoyl paeoniflorin, benzoic acid, catechin, paeonol, gallic acid, and 1,2,3,4,6-penta-O- galloy-D-glucose of Paeoniae Radix Rubra. Methods The mobile phase comprised of acetonitrile and water containing 0.1% phosphoric acid. Column temperature was 30 oC, flow rate was 1.0 mL/min, and chromatography was monitored at 230 nm. Results The correlation coefficients between concentration and chromatographic peak area of gallic acid, oxypaeoniflora, catechin, albiflorin, paeoniflorin, 1,2,3,4,6-penta-O-galloy-D-glucose, benzoic acid, benzoyl paeoniflorin, and paeonol were respectively over 0.999 in the ranges of 0.004-1.200, 0.010-1.500, 0.044-0.660, 0.038-1.140, 0.042-2.100, 0.050-1.250, 0.040-0.600, 0.042-1.260, and 0.004-1.080 mg/mL, with good precision, stability, repeatability, and recovery. Conclusion The method is effective, accurate and reproducible, and can be used for the quality control of Paeoniae Radix Rubra.