Study on steroidal chemical constituents of Physalis minima / 中草药

ABSTRACT

Objective To investigate the steroidal chemical constituents from the whole plants of Physalis minima. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure ODS column chromatography and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical properties and spectral analysis. Results Seven compounds were separated from the whole plant of P. minima, among them, two compounds were identified as physalins and named as 16,24-cyclo-13,14-secoergost-2-ene-18,26- dioicacid-1417,1427-diepoxy-5α,6β,13α,20α,22β,25β-hexahydroxy-1,15-dioxo-γ-lactone-δ-lactone (1) and physalin D (2); The other five compounds were identified as withanlides and named as physagulin L (3), physagulin M (4), physaminimin F (5), physagulin K (6), and withagulatin A (7). Conclusion Compound 1 is a new compound named 25-hydroxy-physalin D, at the same time, compounds 3, 4, and 7 were obtained from this plant for the first time.