Comparative study on populations genetic diversity between wild and cultivated Angelica sinensis / 中草药

ABSTRACT

Objective To compare the populations genetic diversity between wild and cultivated populations of Angelica sinensis. Methods ISSR markers were used to analyze the genetic diversity of 1 038 samples, which included 48 cultivated populations and seven wild populations. Shannon’s information index (I) and other parameters of genetic information were calculated by Popgen 1.32. Using independent sample t-test in SPSS19.0 to examine whether there was significant difference. The AMOVA analysis of the genetic variation of two groups was carried out by using GenAlEx 6.5; UPGMA dendrogram relationship between wild and cultivated populations were clustered by Ntsys, and the correlation of genetic distance and geographic distance was analyzed by using the mental test in GenAlEx 6.5. Results The average percentages of polymorphie bands (PPB) of wild and cultivated populations were 83.77% and 96.10%; Shannon’s information indexes (I) were 0.221 7 and 0.282 8; The average values of Nei’s genetic diversity index (He) were 0.344 4 and 0.434 30, and there had extremely significant differences (P < 0.001). The genetic differentiation coefficient (Gst) and gene flow (Nm) of wild populations were 0.299 1 and 1.171 8, which of cultivated populations were 0.733 4 and 0.181 7. The variation percentage was 15.85% between groups of wild and cultivated. The variation percentage among populations was 54.3%, and the variation percentage within populations was 54.3%; The genetic distance varied from 0.014 4 to 0.730 7. Conclusion The genetic diversity of wild populations was lower than the cultivated populations; The level of genetic differentiation among wild populations was obviously lower than the cultivated populations; The total genetic variation of A. sinensis mainly existed among populations; It has a distant relationship between wild and cultivated populations, and has correlation between genetic distance and geographic distance.