Research on changes of chemical components in Sichuan authentic herbs of Curcuma Phaeocaulis processed with rice vinegar and decocted by detecting based on dynamic variation of pH value / 中草药

ABSTRACT

Objective: To determine the dynamic variation of pH value and seven components including bisdemethoxycurcumin, demethoxycurcumin, curcumin, curdione, curcumol, germacrone and β-elemene during Curcuma Phaeocaulis' processing, in order to study its curcuminoids and volatile oils components in the process of rice vinegar production affected by external factors such as water, heat, and acid. Methods: C. phaeocaulis were processed by using distilled water, 9° rice vinegar, and 9% acetic acid aqueous. The pH value of the decoction at a specific time point in 10 batches was detected. The data were analyzed by SPSS software. The content of seven components in the samples of the raw crude group, the blank group, the vinegar group, and the reference group were determined by HPLC method, and the change of the content was analyzed. Results: Compared with the blank group, the vinegar group and reference group showed low pH value, and there was no significant difference; Compared with the blank group and reference group, the contents of three curcuminoids in the vinegar group increased [the mass fraction of bisdemethoxycurcumin is (0.002 320 ± 0.000 344) mg/g, the mass fraction of demethoxycurcumin is (0.059 65 ± 0.015 64) mg/g, the mass fraction of curcumin is (0.272 5 ± 0.125 2) mg/g], and the contents of volatile oils decreased in different degrees. Conclusion: During the processing progress, the main active constituents of pharmaceutic adjuvant 9° rice vinegar might be acetic acid; The acetic acid and other organic principles of rice vinegar might protect the chemical constituents in C. phaeocaulis by regulating pH value; The toxicity degree of C. phaeocaulis before and after processing with vinegar was regulated by different protecting degrees of different component. It not only reflects the scientificity of 9° rice vinegar as a pharmaceutic adjuvant, but also offers a new method to study the material basis of C. phaeocaulis processing with rice vinegar.