Study on antiplatelet aggregation active components of Erigeron breviscapus in vitro based on spectrum-effect correlation analysis / 中草药

ABSTRACT

Objective: To explore the active components of antiplatelet aggregation of Erigeron breviscapus, chemical fingerprints and bioactivity detection were used to carry out spectrum-effect correlation analysis. Methods: A fingerprinting method was established by ultra-high performance liquid chromatography with ultraviolet detection (UPLC-UV) and then used for fingerprinting of different batches of E. breviscapus. The antiplatelet aggregation biopotency of different batches of E. breviscapus was tested, and the possible active substances were deduced based on spectrum-effect correlation analysis. Furthermore, all five compounds were verified by antiplatelet aggregation in vitro, and the contribution value of relative activity of the five compounds was calculated according to the difference of the contents of five kinds of monomer compounds in E. breviscapus. Results: Through the spectrum-effect correlation analysis of chemical fingerprints and antiplatelet aggregation biopotency of E. breviscapus, five chromatographic peaks with higher bioactivity correlation coefficient were screened and identified, including chlorogenic acid, caffeic acid, scutellarin, isochromic acid A, and ischlorogenic acid C. Further in vitro experiments showed that the five compounds had different levels of antiplatelet aggregation at same concentration (inhibition rate 16.5%-85.5%). The sequence of the relative activity contribution is scutellarin > ischlorogenic acid C > caffeic acid > ischlorogenic acid A > chlorogenic acid. In terms of activity contribution of five compounds, chlorogenic acid C and scutellarin was larger than other compounds. Conclusion: A method for the determination of antiplatelet aggregation biopotency of E. breviscapus in vitro was established. Moreover, scutellarin and ischlorogenic acid C are the main active substances of E. breviscapus in the aspect of antiplatelet aggregation in vitro.