Optimization for prescription of Sanqi Traumatic Ointment by Box-Behnken test design and research on its transdermal permeability in vitro / 中草药

ABSTRACT

Objective To prepare Sanqi Traumatic Ointment (STO) and study its transdermal permeation properties in vitro. Methods In this paper, the appearance description, high-temperature test, low-temperature test and centrifugal test were taken as indexes. Based on the result of a single factor experiment, the formula for the STO was optimized by Box-Behnken test. Franz diffusing cells method was chosen to investigate the tansdermal infiltration capacity of ointment with different mentha camphor penetration enhancers' in vitro, taking dracorhodin, ginsenoside Rg1, asperosaponi VI, and ginsenoside Rb1 as index components. Results The optimal ratio of the prescription was as follows lauryl sodium sulfate-stearyl alcohol-vaseline (1.6126.7227.10). The promoting effect of different concentration of penetration enhancers on dracorhodin, ginsenoside Rg1, ginsenoside VI, and asperosaponin Rb1 penetration were better; The promoting effect of 3% concentration for index component was better, and this preparation had good stability; The transdermal rates of dracorhodin, ginsenoside Rg1, asperosaponi VI, and ginsenoside Rb1 were 3.971, 6.368, 0.415, and 2.780 μg/(cm2∙h).Conclusion STO had a good release and transdermal properties, and transdermal actions were consistent with zero-order kinetics.