Determination of chemical constituents and quantitative fingerprints of Xiao’er Xiaoji Zhike Oral Liquid by HPLC-ESI-Q-TOF/MS / 中草药

ABSTRACT

Objective To establish the HPLC multi-index quantitative fingerprints of Xiao’er Xiaoji Zhike Oral Liquid (XXZOL), and to provide methods and technical support for improving its quality standard. Methods Using an Agilent Zorbax SB-C18 (250 mm × 4.6 mm, 5.0 μm) column, acetonitrile-0.4% glacial acetic acid mobile phase system, gradient elution, flow rate of 0.6 mL/min, column temperature 25 ℃, detection wavelength 254 nm; The HPLC-ESI-Q-TOF/MS technique was used to identify the chemical constituents (peak 1-synephrine, peak 13-chlorogenic acid, peak 16-quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside, peak 22-hyperoside, peak 24-forsythoside A, peak 26-naringin, peak 27-hesperidin, peak 29-neohesperidin, peak 33-phillyrin, peak 37-3,29-dibenzoyl rarounitriol) in the XXZOL, and the content of the main components was determined by HPLC-DAD. The HPLC fingerprint was established on the basis of multi-batch analysis. Results The fingerprints of the 10 batches of samples were established, and the similarities of them were above 0.9, which were 0.999 3, 0.999 6, 0.999 2, 0.999 2, 0.999 4, 0.999 3, 0.999 4, 0.999 5, 0.999 1, and 0.999 7, respectively. XXZOL has 38 common peaks, 32 of them was identified, six of them was unknown; the contents of the 10 chemical constituents were 2 542.42, 158.61, 147.02, 34.35, 432.42, 1 813.01, 1 339.70, 2 580.71, 175.32, and 1 615.17 μg/mL. Conclusion The establishment of multi-index analysis by HPLC and fingerprints of XXZOL provides a scientific basis for improving its quality standards.