Screening and mechanism of anti-inflammatory ingredients in compound Mango leaves formula / 中草药

ABSTRACT

Objective To investigate the anti-inflammatory activity of compound Mango leaves formula (CMLF) and illuminate the network regulated mechanism. Methods UPLC-Q/TOF-MS combined with NF-κB dual-luciferase reporter assay systems was used to screen the anti-inflammatory ingredients in CMLF, network pharmacology approach was utilized to forecast the targets and pathways, and then the anti-inflammatory effect was verified by TNF-α induced BEAS-2B cells inflammatory model and ELISA. Results Sixteen anti-inflammatory ingredients were screened out, and there were 40 targets working on 11 pathways respectively. Conclusion The anti-inflammatory multiple regulation mechanism of CMLF is related to MAPK, focal adhesion.